Application and Cautions
- High potential for operator error, which might result in animal suffering.
- If incorrectly performed the animal may be injured and not either stunned or killed (EFSA, 2005).
- Concussion should be followed by the physical destruction of brain tissue by pithing or crushing the brain (CCAC, 2005).
- Usually preceded by anesthesia to quiet the fish (Kreiberg, 2000).
- The procedure must be performed by someone with appropriate training, and should be conducted in an area beyond the sensory range of other animals.
Canadian Council on Animal Care – CCAC (2005) CCAC guidelines on: the care and use of fish in research, teaching and testing, Ottawa ON: CCAC.
European Food Safety Authority Panel on Animal Health and Welfare – EFSA (2005) Opinion of the Scientific Panel on Animal Health and Welfare on a request from the Commission related to "Aspects of the biology and welfare of animals used for experimental and other scientific purposes". European Food Safety Authority Journal 3(12):1-183.
Kreiberg H. (2000) Stress and anesthesia. In: The Laboratory Fish. (Ostrander G., ed.).San Diego CA: Academic Press, pp.503-511.
Potential Influence on Scientific Data
- Damage to brain tissues.
- Barbiturates & Derivatives
- Clove oil (Eugenol)
- Electrical Stunning
- Etomidate & Metomidate
- Inert gases
- Inhalant Anesthetics
- Penetrating Captive Bolt
- TMS (MS222, tricaine)